8:30 - 9:30 AM |
Prof. B. S. Manjunath
Bioimage Informatics, Part 2: Bisque platform for high-throughput, web-based, bioimage analysis
Bisque is a web-based image database system for storing, managing, analyzing and sharing bioimages. This is an open source software infrastructure that integrates image analysis with databases, and supports most existing microscopy image formats, and including 2D, 3D, 4D and 5D images. In addition to basic functionalities such as viewing, enhancement, and basic analysis modules, users can build and integrate their own modules into Bisque. More information can be found at http://www.bioimage.ucsb.edu
- Introduction to BISQUE
- Loading, annotating, sharing, processing and publishing images in Bisque
References:
Bioimage Website,
Bisque Website.
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9:30 - 10:00 AM |
Tea/Coffee Break
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10:00 - 11:00 am |
Prof. Tao P. Zhong
Part I: Heart Patterning and Morphogenesist
Understanding on the patterning and morphogenesis of embryo and organ development has become a subject of enormous scientific and clinical interest. Zebrafish, which have small, accessible, transparent embryos and larvae, provides a unique living animal model to facilitating high-resolution imaging on embryos and developing organ. Numerous transgenic zebrafish lines have been generated with endogenous fluorescent labeling of developing heart. These animals facilitate high-resolution imaging of individual cardiac cell in vivo and make possible long-term time-lapse imaging of the dynamics of cardiac patterning and morphogenesis. We quantify cardiac cell number using confocal microscopy analysis in Tg(cmlc2:EGFP-nuc) embryos in which a gene encoding fluorescent protein is expressed in nuclei under the control of the cmlc2 promoter. These features make zebrafish particularly well suited for discovering small-molecule regulators of cardiac cell production and regeneration. However, computation algorithms are required to develop to facilitate the automation of cardiac cell number quantification. This may ultimately aid in design of therapeutic approaches for heart regeneration and repair.
Brainbow, a new imaging technology, was recently developed that allowed the designation of 90 colour labels to zebrafish developing heart. With this technology, it was possible to visualize adjacent cardiomyocyte and their connections in the heart with high resolution. The ability to assign many colours to different cardiomyocyte in a population can be applied to investigating cardiomyocyte proliferation, lineage decisions, cardiac patterning and morphogenensis. The large amounts of 3-dimensional imaging data are recognized and analyzed by advanced computation methods.
References
- Ni,T, Rellinger E , Williams C, Stephens L, Hu JY, Kim K, Marnett L, Heaton W, Hatzopoulos A, Zhong TP. 2011. Discovering small molecules that promote cardiomyocyte generation via modulating Wnt signaling. Chemistry & Biology 18,1658-6. [PDF]
- Gupta, V and Poss, KD. 2012. Clonally dominant cardiomyocytes direct heart morphogenesis. Nature 484, 479-48. [PDF]
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11:00 - 12:00 Noon |
Prof. Tao P. Zhong
Part II: Vascular Patterning and Morphogenesis
Understanding on the patterning and morphogenesis of embryo and organ development has become a subject of enormous scientific and clinical interest. Zebrafish, which have small, accessible, transparent embryos and larvae, provides a unique living animal model to facilitating high-resolution imaging on embryos and developing organ. Numerous transgenic zebrafish lines have been generated with endogenous fluorescent labeling of developing heart. These animals facilitate high-resolution imaging of individual cardiac cell in vivo and make possible long-term time-lapse imaging of the dynamics of cardiac patterning and morphogenesis. We quantify cardiac cell number using confocal microscopy analysis in Tg(cmlc2:EGFP-nuc) embryos in which a gene encoding fluorescent protein is expressed in nuclei under the control of the cmlc2 promoter. These features make zebrafish particularly well suited for discovering small-molecule regulators of cardiac cell production and regeneration. However, computation algorithms are required to develop to facilitate the automation of cardiac cell number quantification. This may ultimately aid in design of therapeutic approaches for heart regeneration and repair.
Brainbow, a new imaging technology, was recently developed that allowed the designation of 90 colour labels to zebrafish developing heart. With this technology, it was possible to visualize adjacent cardiomyocyte and their connections in the heart with high resolution. The ability to assign many colours to different cardiomyocyte in a population can be applied to investigating cardiomyocyte proliferation, lineage decisions, cardiac patterning and morphogenensis. The large amounts of 3-dimensional imaging data are recognized and analyzed by advanced computation methods.
References
- Ni,T, Rellinger E , Williams C, Stephens L, Hu JY, Kim K, Marnett L, Heaton W, Hatzopoulos A, Zhong TP. 2011. Discovering small molecules that promote cardiomyocyte generation via modulating Wnt signaling. Chemistry & Biology 18,1658-6. [PDF]
- Gupta, V and Poss, KD. 2012. Clonally dominant cardiomyocytes direct heart morphogenesis. Nature 484, 479-48. [PDF]
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12:00 - 2:00 PM |
Lunch
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2:00 - 3:00 PM |
Prof. Serge Belongie
Visual Recognition With Humans in the Loop
- Overview of Visipedia
- Subordinate vs. Basic Level Categorization
- Human Computation Overview
- The Parts and Attributes Framework
- Decision Trees for Interactive Classification
- Dealing with Noise in User Responses
References: ECCV 2010 Paper, ICCV 2011 Paper, Project Website
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3:00 - 3:30 PM |
Tea/Coffee Break
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3:30 - 4:30 PM |
Prof. Serge Belongie
Crowdsourcing and Its Applications in Computer Vision
- Introduction to Mechanical Turk
- Task Incentives
- Experimental Design
- Quality Management
- Cost Effective Strategies for Obtaining Labels
- Example Applications
References: Report, Presentation Slides, NIPS 2010 Paper, Website
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4:30 - 5:30 PM |
Review and Discussion
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